Journal: Frontiers in Immunology
Article Title: FKBP5-CCL5 interaction promotes neuroinflammation and neuronal apoptosis in ischemic stroke by regulating the MAPK pathway and enhancing NET formation
doi: 10.3389/fimmu.2025.1609989
Figure Lengend Snippet: Increased expression of FKBP5 and NET markers in ischemic stroke patients. Plasma samples and brain tissues were collected within 24 hours from ischemic stroke patients and healthy donors (HDs). (A) FKBP5 is significantly upregulated in the brain tissues in stroke group, a visualization of the sequencing results, n = 5. (B) FKBP5 is significantly upregulated in the brain tissues of expanded cohort in stroke group (n = 10). Levels of H3cit (C) and MPO-DNA complexes (D) in plasma were quantified using ELISA. (E) dsDNA levels in plasma were assessed using the PicoGreen dsDNA Assay kit. n = 40 per group.
Article Snippet: The DNA in plasma was quantified according to the manufacturer’s instructions of the Quant-iT PicoGreen dsDNA Assay kit (12641ES01, Yeasen).
Techniques: Expressing, Clinical Proteomics, Sequencing, Enzyme-linked Immunosorbent Assay, Picogreen Assay